三鍵鍵合的酰胺基(Amide)鍵合相、寬泛的流動相pH條件
自從2003年，沃特世就已經開發出創新的固定相用于親水作用色譜分離，以克服對強極性化合物難以保留和分離的挑戰性問題。基于沃特世新穎BEH顆粒技術，ACQUITY UPLC BEH Amide柱使用化學穩定耐受的、三鍵鍵合的酰胺基(Amide)鍵合相，從而為HILIC分離在穩定性與通用性方面又開創了新維度。
ACQUITY UPLC BEH Amide柱能夠使用寬泛的流動相pH條件(2-11)，從而可以對在極性、分子結構和pKa值性質上跨越大的極性分析物混合物實現優異的保留與分離。
Analysis of Free Bromate Ions in Tap Water using an ACQUITY UPLC BEH Amide Column
SensitivityTo establish reliable data, it is recommended that the analytical method be at least 10-fold more sensitivethan the action limit imposed for the assay. By following the 10 ppb WHO guideline for bromate, this wouldrequire that the analyte be detectable at 1 ppb or lower. Typical IC methods can detect bromate at these verylow levels; however, the methods used for IC are more labor intensive and require a rigorous instrumentmaintenance schedule due to the reagents used for the post-column reaction. In contrast, HILIC chromatography coupled with MS detection provides both ease of use and high sensitivity. Figure 1 shows single ion recording(SIR) MS chromatograms in the range of 0.05 - 1 ppb. The mass-to-charge ratio monitored for the bromate ion was 128.9 Da.
品名 | 編號 |
ACQUITY - XBridge BEH Amide Method Transfer Kit, 130?, 1.7 - 3.5 µm, 2.1 - 4.6 mm X 100 - 150mm, ... | 186004974 |
ACQUITY UPLC BEH Amide Column, 130?, 1.7 µm, 1 mm X 100 mm, 1/pkg | 186004849 |
ACQUITY UPLC BEH Amide Column, 130?, 1.7 µm, 1 mm X 100 mm, 3/pkg | 176001915 |
ACQUITY UPLC BEH Amide Column, 130?, 1.7 µm, 1 mm X 50 mm, 1/pkg | 186004848 |
ACQUITY UPLC BEH Amide Column, 130?, 1.7 µm, 2.1 mm X 150 mm, 3/pkg | 176001909 |
ACQUITY UPLC BEH Amide Method Validation Kit, 130?, 1.7 µm, 2.1 mm X 100 mm, 3/pkg | 186004808 |
ACQUITY UPLC BEH Amide Column, 130?, 1.7 µm, 2.1 mm X 100 mm, 3/pkg | 176001908 |
ACQUITY UPLC BEH Amide Method Validation Kit, 130?, 1.7 µm, 2.1 mm X 50 mm, 3/pkg | 186004807 |
ACQUITY UPLC BEH Amide Column, 130?, 1.7 µm, 3 mm X 150 mm, 1/pkg | 186004806 |
ACQUITY UPLC BEH Amide Column, 130?, 1.7 µm, 3 mm X 100 mm, 1/pkg | 186004805 |
ACQUITY UPLC BEH Amide Column, 130?, 1.7 µm, 3 mm X 50 mm, 1/pkg | 186004804 |
ACQUITY UPLC BEH Amide Column, 130?, 1.7 µm, 3 mm X 30 mm, 1/pkg | 186004803 |
ACQUITY UPLC BEH Amide Column, 130?, 1.7 µm, 2.1 mm X 150 mm, 1/pkg | 186004802 |
ACQUITY UPLC BEH Amide Column, 130?, 1.7 µm, 2.1 mm X 100 mm, 1/pkg | 186004801 |
ACQUITY UPLC BEH Amide Column, 130?, 1.7 µm, 2.1 mm X 50 mm, 1/pkg | 186004800 |
ACQUITY UPLC BEH Amide Column, 130?, 1.7 µm, 3 mm X 30 mm, 3/pkg | 176001910 |
ACQUITY UPLC BEH Amide Method Validation Kit, 130?, 1.7 µm, 2.1 mm X 150 mm, 3/pkg | 186006024 |
ACQUITY UPLC BEH Amide Column, 130?, 1.7 µm, 1 mm X 150 mm, 3/pkg | 176001916 |
ACQUITY UPLC BEH Amide Column, 130?, 1.7 µm, 3 mm X 50 mm, 3/pkg | 176001911 |
ACQUITY UPLC BEH Amide Column, 130?, 1.7 µm, 3 mm X 100 mm, 3/pkg | 176001912 |
ACQUITY UPLC BEH Amide Column, 130?, 1.7 µm, 3 mm X 150 mm, 3/pkg | 176001913 |
ACQUITY UPLC BEH Amide Column, 130?, 1.7 µm, 1 mm X 50 mm, 3/pkg | 176001914 |
ACQUITY UPLC BEH Amide Column, 130?, 1.7 µm, 2.1 mm X 50 mm, 3/pkg | 176001907 |
ACQUITY UPLC BEH Amide Column, 130?, 1.7 µm, 2.1 mm 30 mm, 3/pkg | 176001906 |
ACQUITY UPLC BEH Amide Column, 130?, 1.7 µm, 2.1 mm X 75 mm, 1/pkg | 186005657 |
ACQUITY UPLC BEH Amide Column, 130?, 1.7 µm, 2.1 mm X 30 mm, 1/pkg | 186004839 |
ACQUITY UPLC BEH Amide Column, 130?, 1.7 µm, 3 mm X 75 mm, 1/pkg | 186005658 |
ACQUITY UPLC BEH Amide Column, 130?, 1.7 µm, 1 mm X 150 mm, 1/pkg | 186004850 |
ACQUITY UPLC BEH Amide Method Validation Kit, 130?, 1.7 µm, 3 mm X 50 mm, 3/pkg | 186004809 |
ACQUITY UPLC BEH Amide VanGuard Pre-column, 130?, 1.7 µm, 2.1 mm X 5 mm, 3/pkg | 186004799 |
ACQUITY UPLC BEH Amide Method Validation Kit, 130?, 1.7 µm, 3 mm X 100 mm, 3/pkg | 186004810 |
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